RESOURCES : shRNA

shRNA FAQ

Instructions for Finding shRNA Clones

TRC Sequencing Primers

shRNA Guide/Passenger Strand Generator

Transfection/Transduction Protocol

Click here for the list of shRNA Pathway-specific Panels and Genome-wide Pools

Click on "Map" or "Seq" next to the clone of interest in the list below:

Vectors:

TRC1 (empty)             Seq

TRC2 (empty)             Seq

Lentivirus Packaging Plasmids

pCMV-VSVG   Map   Seq

pDelta8.9         Map   Seq

TRC1 (pLKO.1-puro)

SHC001           Map   Seq

SHC002           Map   Seq

SHC003           Map   Seq

SHC004           Map   Seq

SHC005           Map   Seq

SHC007           Map   Seq
SHC012           Map   Seq

SHC016           Map   Seq

TRC2 (pLKO.5-puro)

SHC201           Map   Seq

SHC202           Map   Seq

SHC203           Map   Seq

SHC204           Map   Seq

Alternate pLKO Vector           Seq

pLKO.1-Puro-CMV-tGFP+shRNA

Map   Seq

pLKO.1-Puro-CMV-tRFP+shRNA

Map   Seq

pLKO.1-Neo-CMV-tGFP+shRNA

Map   Seq

RESOURCES : ORF

ORF FAQ

Instructions for Finding ORF Clones

ORF Sequencing Primers

To view directions for the generation of a pLX304 vector containing your ORF of interest, click here.

RESOURCES : CRISPR-Cas9

CRISPR FAQ (Coming Soon)

NEW Sanger-Sigma Human Whole Genome Arrayed lenti CRISPR Library! For more information click here. 

Sigma Clone Lookup Sheet

Sigma Cas9 plasmids

Sanger Arrayed Library Paper

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gRNA can be custom cloned into Zhang-lab CRISPR vectors. Vector Map can be found on Addgene:

Zhang-lab Vectors:

lentiCRISPRv2

lentiCas9-Blast

lentiCas9-EGFP

lentiGuide-Puro

pSpCas9(BB)-2A-GFP (PX458)

pSpCas9(BB)-2A-Puro (PX459)

pSpCas9n(BB)-2A-GFP (PX461)

pSpCas9n(BB)-2A-Puro (PX462)

lenti sgRNA(MS2)_zeo

lenti MS2-P65-HSF1_Hygro

lenti dCAS-VP64_Blast

sgRNA(MS2)

MS2-P65-HSF1_GFP

RESOURCES: Cell Lines

The Functional Genomics Facility, in collaboration with the Espinosa lab, has generated the following cell lines with human TRC1/1.5 shRNA library. Cell lines were maintained in 1microg/mL of puromycin.

Select a cell line name below to download an Excel spreadsheet containing a list of average reads for individual shRNA clones of TRC1 / 1.5 library. 

Cell Lines:

HCT116

RKO

H460

A549

SJSA