Copyright 2017 Functional Genomics Facility

pCMV-VSVG   Map   Seq

pDelta8.9         Map   Seq

SHC001           Map   Seq

SHC002   Map  Seq

SHC003   Map Seq

SHC012   Map   Seq

SHC016   Map  Seq

SHC202   Map   Seq

SHC203   Map  Seq

TRC1 (empty)   Map   Seq

TRC2 (empty)   Map   Seq

pLKO.1-Neo-CMV-tGFP+shRNA    Map   Seq

pLKO.1-Puro-CMV-tRFP+shRNA   Map   Seq

pLKO.1-Puro-CMV-tGFP+shRNA   Map   Seq

SHC201   Map   Seq

SHC012           Map   Seq

SHC016           Map   Seq

SHC202           Map   Seq

SHC203           Map   Seq

TRC1 (empty)             Seq

TRC2 (empty)             Seq

pLKO.1-Neo-CMV-tGFP+shRNA    Map   Seq

pLKO.1-Puro-CMV-tRFP+shRNA   Map   Seq

pLKO.1-Puro-CMV-tGFP+shRNA   Map   Seq

SHC201           Map   Seq

RESOURCES

RESOURCES : ORF

ORF FAQ

 

Instructions for Finding ORF Clones

 

ORF Sequencing Primers

 

To view directions for the generation of a pLX304 vector containing your ORF of interest, click here.

RESOURCES: Cell Lines

 

 

The Functional Genomics Facility, in collaboration with the Espinosa lab, has generated the following cell lines with human TRC1/1.5 shRNA library. Cell lines were maintained in 1microg/mL of puromycin.

Select a cell line name below to download an Excel spreadsheet containing a list of average reads for individual shRNA clones of TRC1 / 1.5 library. 

 

Cell Lines:

HCT116

RKO

H460

A549

SJSA

 

SHC002           Map   Seq

SHC003           Map   Seq

SHC004           Map   Seq

SHC005           Map   Seq

SHC007           Map   Seq

SHC204           Map   Seq

RESOURCES : CRISPR-Cas9

CRISPR FAQ (Coming Soon)

 

NEW Sanger-Sigma Human Whole Genome Arrayed lenti CRISPR Library!

 

Sigma Clone Lookup Sheet

Sigma Cas9 plasmids

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To view a list of Zhang-lab CRISPR vectors and their descriptions, click here.

 

Click on your vector of interest below to view the Addgene Vector Map:

 

Zhang-lab Vectors:

Vectors:

Alternate pLKO Vector                           Seq

TRC2 (pLKO.5-puro)

TRC1 (pLKO.1-puro)

Lentivirus Packaging Plasmids