August 2015: Moving the Lab
In August of 2015, the Functional Genomics Facility and the Espinosa Lab moved from the Boulder campus to the Department of Pharmacology at the University of Colorado Denver School of Medicine in the Anschutz Medical Campus. Although the move prevented the Facility from fully operating in August, we are excited to reopen for business on a great campus on September 1, 2015! Below are some pictures of the move.
November 2015: Human Chromosome 21 Panel
The Functional Genomics Facility is proud to announce that we are now offering a new gene panel. In light of our director (Dr. Joaquin Espinosa) becoming the Associate Director for Science at the Linda Crnic Institute for Down Syndrome, we now have the Human Chromosome 21 panel available for purchase. We hope this new panel will aid scientists in further researching Down syndrome and discovering treatments that will improve the quality of life for those affected by this condition.
To view the panel, please visit our shRNA Products page.
Click here to learn more about the Linda Crnic Institute for Down Syndrome.
December 2015: Merry CRISPR-mas!
Just in time for the holidays, the Functional Genomics Facility has acquired the Human CRISPR Library! After laboriously extracting the library from hundreds of kilograms of dry ice, we now have the clones stored safely away in our freezers. We are working on updating the Order Form to include this new product. Until then, you can look up your clone of interest in this CRISPR Lookup Sheet, and send us an email titled "FGF Web Order (Your name)" with the clone(s) you'd like to purchase.
For more information regarding the CRISPR Library, view Sigma's flyer here.
Update: You can now view the gRNA library through the link on our Resources page!
There's nothing like a gigantic box of dry ice to make you feel like you're walking in a Winter Wonderland.
March 2016: CRISPR Craze
Are you CRISPRing yet?
Researchers around the world are fast adopting CRISPR-Cas9 to tinker with the genomes of humans, viruses, bacteria, animals and plants. Nature brings together research, reporting and expert opinion to keep you abreast of the frontiers of gene editing. Here are a couple of articles to pique your interest!
April 2016: First Functional Genomics Facility-supported CRISPR-KO published
Yiqun G. Shellman and her team at University of Colorado reports that combining MCL-1 inhibits SC-2001 and ABT-737 is a promising treatment strategy for targeting melanoma. NOXA and BIM were found to be key mediators of this combination-induced cell death.
Molishree Joshi of the Functional Genomics Facility designed and cloned two different guideRNA sequences to target BIM into the lenti-Guide_puro vector. Using these constructs and Cas9_Blast, Dr. Shellman's lab generated multiple clones with complete reduction of BIM protein in melanoma cell lines (See Figure).
Use of a MCL-1 inhibitor alone to de-bulk melanoma and in combination to kill melanoma initiating cells.
May 2016: New Panel - Human Autophagy
In conjugation with the Thorburn Lab at the University of Colorado Anschutz Medical Campus, the Functional Genomics Facility has designed and pooled a new shRNA panel targeting the human autophagy pathway. This panel consists of 935 clones of 199 genes, including built-in non-targeting controls.
To view the complete list of clones, visit our shRNA Products page and click on the Human Autophagy panel.
If you have any questions about the panel, please feel free to contact us!
July 2016: New Staff Members
The Functional Genomics Facility welcomes Mr. Colin Sempeck and Mr. Alex Ho to the Facility!
Mr. Sempeck is joining as a Professional Reserach Assistant to replace Ms. Knox, who is leaving the Facility to begin graduate school. After serving in the U.S. Navy for 6 years, Colin obtained his Bachelor of Science in Biochemistry from Colorado State University in Fort Collins.
Mr. Ho is volunteering as a Research Assistant. He completed his Bachelor of Arts in Molecular, Cellular, and Developmental Biology at the University of Colorado, Boulder, and is currently volunteering in the Facility while preparing for medical school.
To learn more about our new team members, visit our Meet The Team page.
Mr. Colin Sempeck
Mr. Alex Ho
December 2016: CRISPR Holiday Sale!
We are currently offering the Sigma sgRNA Arrayed Library Clones for 50% off of regular prices for the holiday season!
Sigma sgRNA Glycerol stocks are on sale for $63 for Non-Members, and $50 for Members.
Sigma sgRNA Plasmids are on sale for $88 for Non-Members, and $75 for Members.
Sigma sgRNA Lentiviral Suspensions are now $ 100 for Non-Members, and $88 for Members.
These prices are outlined in our updated Product Prices page.
January 2017: Colorado Cancer Blog Post
An article in the CU Cancer Center has been posted about our recent grant to develop custom CRISPR pools for gene knockout! The article, "Grant supports gene editing-based screens at the University of Colorado Cancer Center" outlines our role in functional genomics research, what CRISPR is, and how developing this custom pool program will aid researchers across Colorado! Check it out here!
April 2017: FGF Free to Use Equipment
Howdy all! The FGF is now offering our Lonza 4-D Nucleofector and our Agilent 2100 Bioanalyzer for free use to UC Denver - Anschutz Medical Campus Labs! All you have to do is provide the reagents (nucleofection cuvettes, media, chips, etc.).