Copyright 2017 Functional Genomics Facility

 

CELL LINES

Listed below are cell lines transduced with human TRC library. Excel spreadsheets containing average reads for individual shRNA clones of TRC1 / 1.5 library can be found on the Resources page.

HCT116 is a human colorectal carcinoma (adherent epithelial) cell line. This line has a mutation in codon 13 of the ras proto-oncogene.

 

It was routinely cultured in McCoy’s 5a Medium (M9309; Sigma), 10 % fetal bovine serum and 1X Antibiotic-Antimycotic (Cellgro)

RKO is a poorly differentiated human colon carcinoma (adherent epithelial) cell line.

 

It was routinely cultured in DMEM (D6429; Sigma), 10 % fetal bovine serum and 1X Antibiotic-Antimycotic (Cellgro)

RKO

HCT116

H460

A549

SJSA

H460 is a human large cell lung carcinoma (adherent epithelial) cell line.

 

It was routinely cultured in RPMI-1640 (11875-093; Gibco), 10 % fetal bovine serum and 1X Antibiotic-Antimycotic (Cellgro)

A549 is a human lung carcinoma (adherent epithelial) cell line.

 

It was routinely cultured in DMEM-F12 (11320-033; Gibco), 10 % fetal bovine serum and 1X Antibiotic-Antimycotic (Cellgro)

SJSA is a human osteosarcoma (adherent fibroblast) cell line. This line has amplification of the MDM2 gene, which encodes the p53-associated protein. It also exhibits amplification of the gli proto-oncogene.

 

It was routinely cultured in RPMI-1640 (11875-093; Gibco), 10 % fetal bovine serum and 1X Antibiotic-Antimycotic (Cellgro)